PSIBLAST 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: nr90 38,209,907 sequences; 13,267,535,841 total letters Results from round 1 Query= 3e7u Length=40 Score E Sequences producing significant alignments: (Bits) Value sp|Q53I06|PLECT_PSENR RecName: Full=Plectasin; Flags: Precursor ... 60.8 2e-07 emb|CCX33672.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 60.1 3e-07 emb|CCX33671.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 59.7 4e-07 emb|CCX33670.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 59.7 4e-07 emb|CCX33668.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 57.8 2e-06 >sp|Q53I06|PLECT_PSENR RecName: Full=Plectasin; Flags: Precursor emb|CAI83768.1| defensin [Pseudoplectania nigrella] Length=95 Score = 60.8 bits (146), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 40/40 (100%), Positives = 40/40 (100%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY Sbjct 56 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 95 >emb|CCX33672.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=92 Score = 60.1 bits (144), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 37/40 (93%), Positives = 38/40 (95%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCKSIKGYKGGYCA GFVCKCY Sbjct 53 GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCASAGFVCKCY 92 >emb|CCX33671.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=95 Score = 59.7 bits (143), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 38/40 (95%), Positives = 39/40 (98%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCKSIKGYKGGYCAK GFVCKCY Sbjct 56 GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCAKAGFVCKCY 95 >emb|CCX33670.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=95 Score = 59.7 bits (143), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 36/40 (90%), Positives = 38/40 (95%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCK+IKGYKGGYCA GFVCKCY Sbjct 56 GFGCNGPWDEDDMKCHNHCKTIKGYKGGYCASAGFVCKCY 95 >emb|CCX33668.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=94 Score = 57.8 bits (138), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 36/40 (90%), Positives = 37/40 (93%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGC GPW+EDDMQCHNHCKSIKGYKGGYCA GFVCKCY Sbjct 55 GFGCGGPWNEDDMQCHNHCKSIKGYKGGYCASAGFVCKCY 94 Lambda K H 0.329 0.148 0.617 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 331047525718 Results from round 2 Query= 3e7u Length=40 Score E Sequences producing significant alignments: (Bits) Value Sequences used in model and found again: emb|CCX33671.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 54.8 9e-06 emb|CCX33670.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 53.7 2e-05 sp|Q53I06|PLECT_PSENR RecName: Full=Plectasin; Flags: Precursor ... 53.7 2e-05 emb|CCX33672.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 53.7 2e-05 emb|CCX33668.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema ... 52.9 4e-05 Sequences not found previously or not previously below threshold: >emb|CCX33671.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=95 Score = 54.8 bits (131), Expect = 9e-06, Method: Composition-based stats. Identities = 38/40 (95%), Positives = 39/40 (98%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCKSIKGYKGGYCAK GFVCKCY Sbjct 56 GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCAKAGFVCKCY 95 >emb|CCX33670.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=95 Score = 53.7 bits (128), Expect = 2e-05, Method: Composition-based stats. Identities = 36/40 (90%), Positives = 38/40 (95%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCK+IKGYKGGYCA GFVCKCY Sbjct 56 GFGCNGPWDEDDMKCHNHCKTIKGYKGGYCASAGFVCKCY 95 >sp|Q53I06|PLECT_PSENR RecName: Full=Plectasin; Flags: Precursor emb|CAI83768.1| defensin [Pseudoplectania nigrella] Length=95 Score = 53.7 bits (128), Expect = 2e-05, Method: Composition-based stats. Identities = 40/40 (100%), Positives = 40/40 (100%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY Sbjct 56 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 95 >emb|CCX33672.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=92 Score = 53.7 bits (128), Expect = 2e-05, Method: Composition-based stats. Identities = 37/40 (93%), Positives = 38/40 (95%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGCNGPWDEDDM+CHNHCKSIKGYKGGYCA GFVCKCY Sbjct 53 GFGCNGPWDEDDMKCHNHCKSIKGYKGGYCASAGFVCKCY 92 >emb|CCX33668.1| Similar to Plectasin; acc. no. Q53I06 [Pyronema omphalodes CBS 100304] Length=94 Score = 52.9 bits (126), Expect = 4e-05, Method: Composition-based stats. Identities = 36/40 (90%), Positives = 37/40 (93%), Gaps = 0/40 (0%) Query 1 GFGCNGPWDEDDMQCHNHCKSIKGYKGGYCAKGGFVCKCY 40 GFGC GPW+EDDMQCHNHCKSIKGYKGGYCA GFVCKCY Sbjct 55 GFGCGGPWNEDDMQCHNHCKSIKGYKGGYCASAGFVCKCY 94 Lambda K H 0.329 0.156 0.665 Gapped Lambda K H 0.267 0.0473 0.140 Effective search space used: 331047525718 Search has CONVERGED! Database: nr90 Posted date: Apr 26, 2016 10:28 PM Number of letters in database: 13,267,535,841 Number of sequences in database: 38,209,907 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40