ID AB031077; SV 1; circular; other DNA; STD; SYN; 5680 BP. XX AC AB031077; XX DT 14-JAN-2000 (Rel. 62, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pMG103 DNA, complete sequence. XX KW . XX OS Cloning vector pMG103 OC other sequences; artificial sequences; vectors. XX OS synthetic construct OC other sequences; artificial sequences. XX OS Rhodopseudomonas palustris OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bradyrhizobiaceae; Rhodopseudomonas. OG Plasmid pMG101 XX RN [1] RP 1-5680 RA Inui M.; RT ; RL Submitted (16-AUG-1999) to the INSDC. RL Masayuki Inui, Research Institute of Innovative Technology for the Earth, RL Molecular Microbiology and Genetics Laboratory; 9-2, Kizugawadai, Soraku, RL Kizu, Kyoto 619-0292, Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, RL Fax:81-774-75-2321) XX RN [2] RX DOI; 10.1128/AEM.66.1.54-63.2000. RX PUBMED; 10618203. RA Inui M., Roh J.H., Zahn K., Yukawa H.; RT "Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas RT palustris and construction of stable cloning vectors"; RL Appl. Environ. Microbiol. 66(1):54-63(2000). XX FH Key Location/Qualifiers FH FT source 1..5680 FT /organism="Cloning vector pMG103" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:102152" FT source 1..27 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG298" FT /db_xref="taxon:32630" FT source 28..3080 FT /organism="Rhodopseudomonas palustris" FT /plasmid="pMG101" FT /mol_type="other DNA" FT /note="derived from 3.0-kb XhoI-SalI fragment of pMG101" FT /db_xref="taxon:1076" FT source 3081..5680 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG298" FT /db_xref="taxon:32630" FT CDS 3331..4146 FT /codon_start=1 FT /transl_table=11 FT /product="aminoglucoside phosphotransferase" FT /note="kanamycin-resistance" FT /protein_id="BAA89407.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS complement(4147..4455) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ alpha" FT /protein_id="BAA89408.1" FT /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT misc_feature 4385..4441 FT /note="multiple cloning sites" XX SQ Sequence 5680 BP; 1278 A; 1452 C; 1604 G; 1346 T; 0 other; gaggtctgcc tcgtgaagaa ggtgttgctc gagcgggtca tgaacaccct gccagatcga 60 gaatcccgaa cttggcgcgc ggtattcttc aaggccctgc ctgaggtgat ggaggatttt 120 gggggcgact ggctacaagc aactggcgaa cggcgaaatc cgttcgagcg ggtggaacag 180 acgaagccaa taggaaggcc ataggcgcgt ttttatagac ttgaagcccg gaagtccgtc 240 cacacccaaa aaatcgctgt gcgaggcgct gtggctgtcc tgtgttgcct tcgatgaaag 300 gcgaaacttg ccctcatcat ggcagtcccc cctagtctgg tccttaaagc ggtcaagggc 360 gatgccggag ggagcccttg agggcttgtg gggactaggc ttctttccct ttttcaatcg 420 tctttttgaa gactgcttgt tctgaagatt ctgaaaagtg ttaggaagaa gtgttctgca 480 atgttctgta gtgttctgtt ctatatagcg ccaccaagtc tgcggtagag cgctaccaag 540 tctgcggaaa gccgccacca agtctgcggt agaagaagcg aaaacgccac caagtctgcg 600 gtagagtgcc accaagtctg cggtggcggc ggaataagcc cttgaaatta ttgagtcccg 660 ggaacagtaa ggcgccacca agtctgcgga gctaagtggt tgcgtttgcg gctacttttt 720 gcgcacacgg atcgcaggta atgccagcac ggcggggtcg gctggttcga tggaaatgcc 780 gtattgcgtg agcttgagag gcagctttgg cagcacttcc ttgatgtgtg tcagctcctc 840 gatcagctgc gcccggaacg cgcgaggccg agtgaaacct tggccaaatt gttcgctgag 900 agccttccaa ctcatggtca ccgggtggcg aatattgtga aggcgatagc ctagccagaa 960 atacaaatcg agcttgcgag ctgagcctgc gaaggctcga accgcattag cattcaaggg 1020 gagcgcacgc tgctgtaggc tctcaaagaa taccgggttg aacgtgacgg tactgggcca 1080 aagcgagcgt tggtcaagat gcggcggtag ccacacctca acctcatcga acggattgac 1140 ggttttggtt tttgccctca ccccgtccca cacactcacc cgcattgtgc aagccgccag 1200 tgcgttaatt tgttctttga aggcggtgag cgggcctttg cgtcccccgc tgtccgaaaa 1260 tcccatctcg cgcacaaagg cagtaaagct ctcggcgatt tcgatagtag gtgttttctg 1320 ccggatggct tccgaacaaa ggtgcatcat caccagtcgg gcttttggcc cgaaaggcag 1380 cggttgaagg attttcttgc cttctccgtc tcgcaggaat ccggcggaca aatccagcgc 1440 catattgccc tgcttccgtt cgaactcgcg cacttcgaga ggttgacggt cgtaaggcat 1500 accgcaaacg gcaagcacgc tgtgcaaatg ccgaacgtcg gcgggcgatg gcggtgagtt 1560 ttcgataact tcgcggatga tggaacggcg gcggcgctcg cgtggcattg cgtcaagctc 1620 agccttgcgc ttttcagcgg cggcctgttc ctcgtctcgc ttttgctggc gggtgatgag 1680 cacgctgact tgggcatcaa acgcaaactt accccgcgct ttttcaagct cggcgcgcaa 1740 atcttcatcc cggatcagcg attcgtcata cttgcccata ggcacagagt agctgatttg 1800 ctgcggtctc cctaggcgcc tgcctgccta tagggcaatt acgtacagca ttatagcgtt 1860 gtggcgttgt aacgctatag cattgcaatg ctaggacttg cgcttggccg tctcggagtg 1920 gccgcgccta gcaatcacag catcgactcc ctcgacgatg aggtcatgca cctttttgcg 1980 ctcgacaaag gcgatctctc gcagccgatc atgagccgct tggccaaata gatactggag 2040 cgcactacag gagaggcttc agatggggga ggagcggctt ttggcgcgcg catcttcacc 2100 accctggatt tcgacggagc cggagcgtca ggctccacct cgggggtgat ttctagccct 2160 tcgatgatag gggggcgctt tttcggcggg ctcatgcgac ggctttcgtt ttgagtttgt 2220 gctggatgga tctccagagt ccgcgggcct cctcggccgc cttgccgctg ggagcgtact 2280 cggtgacccc ctgccctgcc gcgatggcat cctgaaagtc agccctagaa accatcaatg 2340 gctctgcaag tgatcccagg gctgacaatc ccacggcggc ctcgctggcg cgcccgctgc 2400 gggtgatcgg ggggcactgg ttgagaacaa acaggaatgg ccgctgcagc ttgagaagcg 2460 cttggatggt cggccgggtt gcctgaatgt ctaggcgcgt cggcctagca ggcactaggg 2520 agaggtcggc tgcctgcatg gcgagagagg tggcggtgct ggcgacaccc gggcaatcga 2580 ggatggcgag ggtgtacccc ttccctgcca gcgccttcag gatttgaggg agctggggca 2640 ccttgtcagg cggaagggca tcaacggcgg gcttctcccg ctgggcctga gcccgttgat 2700 ctccccaagc tgcaagcgaa ccttgcgggt caaggtcgag ggcaatcacg gattcccctg 2760 cctctgtggc cgcgacggca atggcagcag cgagggtagt ttttcccgcg ccgcccttct 2820 gcgtgaccag agcaattgtc ttcatgcctg cactatagca ttaaggcact aaagcgtcaa 2880 agcgccatag cggcatagcg gcatagcggc atagcgctaa aatgctatag cattattaaa 2940 tacagcgcta cagcgctata atgctgcaac ggttaggacc gcaatttgcg ccccgggccg 3000 gttgcgctat cgaccagctc aattaactgc tcgggctcgg acgcgaacca cgcgaagctg 3060 ccccaagcca aggagtcgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag 3120 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg 3180 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa 3240 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 3300 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc 3360 tcgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 3420 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca 3480 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 3540 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 3600 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta 3660 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 3720 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 3780 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 3840 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg 3900 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 3960 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 4020 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 4080 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 4140 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 4200 gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg 4260 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 4320 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 4380 tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt 4440 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4500 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4560 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4620 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgaa cttttgctga 4680 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa 4740 gttcaaaatc agtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca 4800 acattgaaac gctgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg agcttcttcc 4860 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 4920 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 4980 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5040 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5100 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5160 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5220 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5280 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5340 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5400 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 5460 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5520 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5580 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5640 ttttctacgg ggtctgacgc tcagtggaac gatccgtcga 5680 //