Silent file is a compact format file which stores information from multiple structures. Silent files can be generated by many Rosetta simulations. Such as Abintio and Ligand Docking. You also can use silent file tools to combine regular pdb files to be binary format silent file to save computer storage spaces. We will briefly introduce silent file format here.

Protein Silent Struct is usually seen in the Abinitio outputs.

• Header

  SEQUENCE                      Structure sequences presented by one letter amino acid code
  SCORE                         Rosetta score terms
  

• Body

  Colunms 1-4                   Residue sequence number
  Colunms 5-7                   Seconary structure one letter code
  Colunms 8-17                  Phi angle
  Colunms 18-26                 Psi angle
  Colunms 27-35                 Omega angle
  Colunms 36-44                 CA atom coordinates x
  Colunms 45-53                 CA atom coordinates y
  Colunms 55-62                 CA atom coordinates z
  Colunms 64-98                 Chi angle real data if possible
  

Binary Silent Struct File is very useful to compress multiple pdbs and save computer spaces.

• Header

  SEQUENCE                      Structure sequences presented by one letter amino acid code
  SCORE                         Rosetta score terms
  

• Body

  Binary contents